Homing Endonucleases: Methods and Protocols by David R. Edgell (English) Paperba

Description: Homing Endonucleases by David R. Edgell Homing Endonucleases: Methods and Protocols aims at providing molecular biologists with a comprehensive resource to identify and characterize homing endonucleases from genomic sequence, to deduce the biological basis of binding and cleavage specificity, as well as to provide protocols to redesign endonuclease target specificity for genome-editing applications. Engineering of designer homing endonucleases has set the stage for genome editing of complex eukaryotic genomes with a broad range of potential applications including targeted gene knockouts in model organisms and gene therapy in humans, making this book a valuable resource for future research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Homing Endonucleases: Methods and Protocols serves as a key reference for all labs studying site-specific DNA endonucleases. FORMAT Paperback LANGUAGE English CONDITION Brand New Back Cover Homing Endonucleases: Methods and Protocols aims at providing molecular biologists with a comprehensive resource to identify and characterize homing endonucleases from genomic sequence, to deduce the biological basis of binding and cleavage specificity, as well as to provide protocols to redesign endonuclease target specificity for genome-editing applications. Engineering of designer homing endonucleases has set the stage for genome editing of complex eukaryotic genomes with a broad range of potential applications including targeted gene knockouts in model organisms and gene therapy in humans, making this book a valuable resource for future research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Homing Endonucleases: Methods and Protocols serves as a key reference for all labs studying site-specific DNA endonucleases." Table of Contents Homing Endonucleases: From Genetic Anomalies to Programmable Genomic Clippers.- Bioinformatic Identification of Homing Endonucleases and Their Target Sites.- PCR-Based Bioprospecting for Homing Endonucleases in Fungal Mitochondrial rRNA Genes.- Mapping Homing Endonuclease Cleavage Sites Using In Vitro Generated Protein.- Mapping Free-Standing Homing Endonuclease Promoters Using 5RLM-RACE.- PCR Analysis of Chloroplast Double-Strand Break (DSB) Repair Products Induced by I-CreII in Chlamydomonas and Arabidopsis.- A Two-Plasmid Bacterial Selection System for Characterization and Engineering of Homing Endonucleases.- Rapid Screening of Endonuclease Target Site Preference Using a Modified Bacterial Two-Plasmid Selection.- A Yeast-Based Recombination Assay for Homing Endonuclease Activity.- Rapid Determination of Homing Endonuclease DNA Binding Specificity Profile.- Quantifying the Information Content of Homing Endonuclease Target Sites by Single Base Pair Profiling.- Homing Endonuclease Target Site Specificity Defined by Sequential Enrichment and Next-Generation Sequencing of Highly Complex Target Site Libraries.- Homing Endonuclease Target Determination Using SELEX Adapted for Yeast Surface Display.- Engineering and Flow-Cytometric Analysis of Chimeric LAGLIDADG Homing Endonucleases from Homologous I-OnuI-Family Enzymes.- Bioinformatics Identification of Coevolving Residues.- Identification and Analysis of Genomic Homing Endonuclease Target Sites.- Redesigning the Specificity of Protein-DNA Interactions with Rosetta. Long Description Homing Endonucleases: Methods and Protocols aims at providing molecular biologists with a comprehensive resource to identify and characterize homing endonucleases from genomic sequence, to deduce the biological basis of binding and cleavage specificity, as well as to provide protocols to redesign endonuclease target specificity for genome-editing applications. Engineering of designer homing endonucleases has set the stage for genome editing of complex eukaryotic genomes with a broad range of potential applications including targeted gene knockouts in model organisms and gene therapy in humans, making this book a valuable resource for future research.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Homing Endonucleases: Methods and Protocols serves as a key reference for all labs studying site-specific DNA endonucleases. " Feature Serves as a comprehensive resource to identify and characterize homing endonucleases and much more Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Details ISBN1493963198 Publisher Humana Press Inc. Year 2016 ISBN-10 1493963198 ISBN-13 9781493963195 Format Paperback Language English Media Book DEWEY 572.8 Imprint Humana Press Inc. Subtitle Methods and Protocols Place of Publication Totowa, NJ Country of Publication United States Pages 284 Edited by David R. Edgell Short Title Homing Endonucleases Series Number 1123 AU Release Date 2016-08-23 NZ Release Date 2016-08-23 US Release Date 2016-08-23 UK Release Date 2016-08-23 Author David R. Edgell Birth 1972 Affiliation Solicitor (Non-Practising) Senior Lecturer (Part Time) Staffordshire University Position Professor of Comparative Literature Edition Description Softcover reprint of the original 1st ed. 2014 Series Methods in Molecular Biology Publication Date 2016-08-23 Alternative 9781627039673 Audience Professional & Vocational Illustrations 39 Illustrations, color; 21 Illustrations, black and white; X, 284 p. 60 illus., 39 illus. in color. We've got this At The Nile, if you're looking for it, we've got it. With fast shipping, low prices, friendly service and well over a million items - you're bound to find what you want, at a price you'll love! TheNile_Item_ID:100253205;

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