Rational Design of Stable Protein Formulations: Theory and Practice by John F. C

Description: Rational Design of Stable Protein Formulations by John F. Carpenter, Mark C. Manning Recombinant proteins and polypeptides continue to be the most important class of biotechnology-derived agents in pharmaceutical industry. This book describes the level of understanding of protein instability and the strategies for stabilizing proteins under a variety of stressful conditions. FORMAT Hardcover LANGUAGE English CONDITION Brand New Publisher Description Recombinant proteins and polypeptides continue to be the most important class of biotechnology-derived agents in todays pharmaceutical industry. Our fundamental understanding of how proteins degrade and how stabilizing agents work has made it possible to approach the formulation of protein pharmaceuticals from a much more rational point of view. This book describes the current level of understanding of protein instability and the strategies for stabilizing proteins under a variety of stressful conditions. Table of Contents 1 Practical Approaches to Protein Formulation Development.- Preparation for Formulation Development.- Preformulation Development.- Formulation Development.- Formulation in Commercial Product Development.- Appendix: List of Regulatory Documents.- References.- 2 Recombinant Production of Native Proteins fromEscherichia coli.- Distribution of Expressed Proteins.- Cell Washing and Lysis.- Purification of Soluble, Folded Proteins.- Purification and Refolding of Soluble, Misfolded Proteins.- Purification and Refolding of Proteins from Inclusion Bodies.- Refolding Mechanism.- Methods to Analyze Folded Structures.- References.- 3 Physical Stabilization of Proteins in Aqueous Solution.- Overview of Physical Stability.- Interactions of Excipients with Proteins.- Physical Factors Affecting Protein Stability.- Conclusions.- Appendix: Derivation of the Wyman Linkage Function and Application to the Timasheff Preferential Exclusion Mechanism.- References.- 4 Effects of Conformation on the Chemical Stability of Pharmaceutically Relevant Polypeptides.- Relationship Between Structure and Deamidation Rates.- Role of Structure in Protein Oxidation.- Summary.- References.- 5 Rational Design of Stable Lyophilized Protein Formulations: Theory and Practice.- Minimal Criteria for a Successful Lyophilized Formulation.- Rational Design of Stable Lyophiilized Formulations.- Acknowledgments.- References.- 6 Spray-Drying of Proteins.- Introduction: Why Spray-Dry a Protein?.- Developments in the Last 10 Years.- The Practice of Spray-Drying Proteins.- Concluding Remarks.- References.- Chapter7 Surfactant-Protein Interactions.- Proteins and Surfactants at Surfaces.- Protein-Surfactant Interactions in Solution.- Surfactant Effects on Protein Assembly State.- Surfactant Effects on Proteins During Freezing, Freeze-Drying and Reconstitution.- Enzymatic Degradation of Non-Ionic Surfactants.- Recommendations for Protein Formulation.- References.- 8 High Throughput Formulation: Strategies for Rapid Development of Stahle Protein Products.- Overall Structure of the HTF Approach.- Role of an Established Decision Tree for Formulation Design.- Use of Software and Databases to Assist in the HTF Process.- Essential Analytical Methods.- Stability Protocols.- Unified Strategy for HTF.- References. Promotional Springer Book Archives Long Description Recombinant proteins and polypeptides continue to be the most important class of biotechnology-derived agents in todays pharmaceutical industry. Over the past few years, our fundamental understanding of how proteins degrade and how stabilizing agents work has made it possible to approach formulation of protein pharmaceuticals from a much more rational point of view. This book describes the current level of understanding of protein instability and the strategies for stabilizing proteins under a variety of stressful conditions. Details ISBN0306467410 Author Mark C. Manning Short Title RATIONAL DESIGN OF STABLE PROT Series Pharmaceutical Biotechnology Language English ISBN-10 0306467410 ISBN-13 9780306467417 Media Book Format Hardcover DEWEY 615.19 Series Number 13 Year 2002 Publication Date 2002-04-30 Subtitle Theory and Practice Edited by John F. Carpenter Place of Publication New York Country of Publication United States Imprint Kluwer Academic/Plenum Publishers Pages 206 Edition 2002nd DOI 10.1007/b112931;10.1007/978-1-4615-0557-0 AU Release Date 2002-04-30 NZ Release Date 2002-04-30 US Release Date 2002-04-30 UK Release Date 2002-04-30 Publisher Springer Science+Business Media Edition Description 2002 ed. Alternative 9781461351313 Illustrations XVII, 206 p. Audience Undergraduate We've got this At The Nile, if you're looking for it, we've got it. With fast shipping, low prices, friendly service and well over a million items - you're bound to find what you want, at a price you'll love! TheNile_Item_ID:96232072;

Price: 317.54 AUD

Location: Melbourne

End Time: 2025-01-15T00:59:34.000Z

Shipping Cost: 17.18 AUD